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Types of Carbohydrates

Carbohydrates are classified generally according to their degree of complexity. Hence, the free sugars such as glucose and fructose are termed monosaccharides; sucrose and maltose, disaccharides; and the starches and celluloses, polysaccharides. Carbohydrates of short chain lengths such as raffinose, stachyose and verbascose, which are three, four and five sugar polymers respectively, are classified as oligosaccharides.

1 Pentoses
Pentoses are five-carbon sugars seldom found in the free state in nature. In plants they occur in polymeric forms and are collectively known as pentosans. Thus, xylose and arabinose are the constituents of pentosans present in plant fibres and vegetable gums, respectively. As the sugar moieties in nucleic acids and riboflavin, ribose and deoxyribose are indispensable constituents of the life process. D-ribose has the following chemical structure:
2 Hexoses
The hexoses comprise a large group of sugars. Principal among these are: glucose, fructose, galactose and mannose. While glucose and fructose are found free in nature, galactose and mannose occur only in combined form. The hexoses are divided into aldoses and ketoses according to whether they possess aldehydic or ketonic groups. Thus, glucose is an aldo sugar and fructose is a keto sugar. The presence of aymmetric centres in all sugars with three or more carbon atoms gives rise to stereoisomers. Galactose and mannose are stereoisomers of glucose which, theoretically, is only one of 16 stereoisomers. Because the ketohexoses have only three asymmetric centres, fructose is one of eight stereoisomers. The chemical configurations of the four hexoses mentioned are as follows:
A general phenomenon, known as mutarotation, is observed in a variety of pentoses and hexoses as well as in certain disaccharides. For example, it has been established that two isomers of D-glucose exist, hence requiring an additional asymmetric centre in this sugar. It became apparent that D-glucose and most other sugars have cyclic structures. The position of the hydroxyl group in relation to the ring oxygen characterizes this additional configurations modification. By convention, the positioning of the hydroxyl group on carbon atom 1 on the same side of the structure as the oxygen ring describes a -modification; and, the positioning of the same hydroxyl group on the opposite side of the ring oxygen describes a b -modification.
Carbohydrases, which catalyse the hydrolysis of glycosidic linkages of simple glycosides, oligosaccharides and polysaccharides often exhibit specificity with regard to substrate configuration. As we shall see later, the specificity for enzyme hydrolysis of certain oligosaccharides helps to explain the poor utilization of this class of carbohydrates in fish nutrition.

Sugars containing the aldo or the keto group are capable of reducing copper in alkaline solutions (Fehling's solution) to produce the brick-red colouration of cuprous ions. These sugars are called reducing sugars and the reaction, although not specific for reducing sugars, has use for both qualitative and quantitative determinations.

Glucose is widely distributed in small amounts in fruits, plant juices and honey. It is commercially produced by the acid or enzyme hydrolysis of grain and root starches. Glucose is of special interest in nutrition because it is the end-product of carbohydrate digestion in all non-ruminant animals including fish.

Fructose is the only important ketohexose and is found in the free state alongside glucose in ripening fruits and honey. Combined with glucose it forms sucrose. Fructose is somewhat sweeter than sucrose and is produced in increasing quantities commercially as a sweetener.

Galactose occurs in milk in combination with glucose. It is also present in oligo-saccharides of plant origin, in combination with both glucose and fructose.

Mannose is present in some plant polysaccharides collectively termed mannans.

3 Disaccharides
Disaccharides are condensation products of two molecules of monosaccharides. Sucrose is the predominant disaccharide occurring in the free form and is the principal substance of sugar cane and sugar beet. It is also formed during germination of legume seeds. Other common disaccharides are maltose and lactose. Maltose is a dimer of glucose, and lactose is a copolymer of galactose and glucose. The two molecules of glucose in maltose are held together in an a -1,4 glycosidic linkage whereas the two hexose entities of galactose are linked at the b -1,4 position. Glucose and fructose are combined in an a -1,2 linkage in sucrose. The abbreviated name of sucrose is D-Glu-(a, 1® 2)-D-Fru.
4 Oligosaccharides
The Oligosaccharides raffinose, stachyose and verbascose are present in significant quantities in legume seeds. Raffinose, which is the most widespread among the three, consists of one molecule of glucose linked to a molecule of sucrose at the a -1,6 position. Its abbreviated chemical name is a -D-Gal (1® 6) -a - D -Glu - (1® 2) - b -D-Fru. Further chain elongation at the galactose end with another galactose molecule will yield stachyose. These galactose-galactose linkages are all at the a-l,6 position and digestion of these Oligosaccharides by animals requires a highly specific enzyme not elaborated by the animals themselves but by certain bacteria present in the animals guts. The gradual disappearance of oligosaccharides from the cotelydons of legume seeds during germination is part of an intricate process beginning with uptake of water by the seed. This uptake of moisture releases gibberellic acid which in turn activates the DNA in the seed, thereby triggering the life cycle of the plant. The DNA directs the production of a -galactosidase which is required for the hydrolysis of these Oligosaccharides. Any interference of the DNA transcription process blocks enzyme production and will be evidenced by continued senescence of the seed and persistence of oligosaccharides in the seed cotelydons.

2.5 Polysaccharides
The polysaccharides represent a large group of complex carbohydrates which are condensation products of undetermined numbers of sugar molecules. The various subgroups are rather ill-defined and there is a lack of agreement on their classification. Most polysaccharides are insoluble in water. Upon hydrolysis with acids or enzymes they eventually yield their constituent monosaccharides.

Starch is a high molecular weight polymer of D-glucose and is the principal reserve carbohydrate in plants. Most starches consist of a mixture of two types of polymers, namely; amylose and amylopectin. The proportion of amylose and amylopectin is generally one part of amylose and three parts of amylopectin. Enzymes capable of catalyzing the hydrolysis of starch are present in the digestive secretions of animals and fish within their cells. The a-amylases which are found virtually in all living cells cleave the a -D-(1® 4) linkages at random and bring about an eventual total conversion of the starch molecule into the reducing sugars. The principal a -amylases of animal origin are those produced in the salivary gland and the pancreas. Starch is insoluble in water and is stained blue by iodine.

Glycogen is the only complex carbohydrate of animal origin. It exists in limited quantities in liver and muscle tissues and acts as a readily available energy source.

Dextrins are intermediate compounds resulting from incomplete hydrolysis or digestion of starch. The presence of a -D-(1® 6) linkages in amylopectin and the inability of a -amylase to cleave these bonds give rise to low molecular weight carbohydrate segments called limit dextrins. These residues are acted upon primarily by acidophilic bacteria in the digestive tract.

Cellulose is made up of long chains of glucose units held together by b -D-(1® 4) linkages. The enzymes which cleave these linkages are not ordinarily present in the digestive secretions of animals and fish although some species of shellfish are believed to elaborate cellulase, the enzyme which catalyzes the hydrolysis of cellulose. Cellulase producing micro-organisms present in the gut of herbivorous animals and fish impart to their host animals the ability to utilize as food the otherwise indigestible cellulose.

Other complex polysaccharides in common occurrence are the hemicelluloses and pentosans. Hemicellulose represents a group of carbohydrates including araban, xylan, certain hexosans and polyuronides. These substances are generally less resistant to chemical treatment and undergo some degree of enzymatic hydrolysis during normal digestive processes. Pentosans are polymers of either xylose or arabinose as constituents of plant structural material and vegetable gums, respectively.

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